Journal: Discover. Oncology

Article Title: miR-17 ~ 92 suppresses proliferation and invasion of cervical cancer cells by inhibiting cell cycle regulator Cdt2

doi: 10.1007/s12672-023-00775-3

Figure Lengend Snippet: miR-17 ~ 92 over expression decreases Cdt2 expression in cervical cancer cells. A RT-qPCR analysis of mRNA level of Cdt2, 48 h after transfection of miR-17 ~ 92 in HEK293T, C33A and SiHa cell lines B Western blot for analysis of Cdt2 protein expression level, 48 h after transfection of miR-17 ~ 92 in HEK293T, SiHa, HeLa and C33A cell lines. C Quantification of Cdt2 protein level, 48 h after miR-17 ~ 92 transfection in HEK293T, SiHa, HeLa and C33A cell lines compared to vector control. The experiments were done in triplicates. Error bar depicts standard error (S.E.). *p value < 0.05 and **p value < 0.001

Article Snippet: C33A (HPV negative cervical cancer cell line) and HEK293T (Human Embryonic Kidney cell line) were procured from National Centre for Cell Science (NCCS, Pune, India).

Techniques: Over Expression, Expressing, Quantitative RT-PCR, Transfection, Western Blot, Plasmid Preparation

Journal: Discover. Oncology

Article Title: miR-17 ~ 92 suppresses proliferation and invasion of cervical cancer cells by inhibiting cell cycle regulator Cdt2

doi: 10.1007/s12672-023-00775-3

Figure Lengend Snippet: miR-17 ~ 92 was ectopically expressed and the proliferation was observed from day of transfection till day 4. A Growth curve of HEK293T cells with its respective control. B Control and treated HEK293T cells after 48 h of transfection under phase contrast microscopy. C Growth curve of SiHa cells along with its respective control D Control and treated SiHa cells under phase contrast microscopy after 48 h of transfection. E Growth curve of HeLa and its respective control. F Control and treated HeLa cells after 48 h of transfection under phase contrast microscopy. G Growth curve of C33A cells and its respective control. H Control and treated C33A cells after 48 h of transfection under phase contrast microscopy. The experiment was done in triplicate. Error bar represents S.D. *p value- < 0.05, **p value < 0.001

Article Snippet: C33A (HPV negative cervical cancer cell line) and HEK293T (Human Embryonic Kidney cell line) were procured from National Centre for Cell Science (NCCS, Pune, India).

Techniques: Transfection, Microscopy